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quick coating solution  (Angio-Proteomie)


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    Structured Review

    Angio-Proteomie quick coating solution
    Quick Coating Solution, supplied by Angio-Proteomie, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quick+coating+solution/Quick+Coating+Solution/pm41688407-236-198-202
    Average 95 stars, based on 61 article reviews
    quick coating solution - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Mechanical, Biochemical, and Multicellular Effects on Vessel Network Morphometrics in a Microfluidic Vasculature-on-a-Chip
    Article Snippet: .. Red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs, cAP-0001RFP) and green fluorescent protein-expressing human adult lung fibroblasts (GFP-hLFs, cAP-0033GFP) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured on T75 flasks coated with Quick Coating solution (cAP-01, Angio-Proteomie) according to manufacturer’s protocol. .. RFP-HUVECs were cultured in EGM-2 media with EGM-2 BulletKit supplements (CC-3162, Lonza) and used between passages 6 to 7.

    Article Title: CLIC5A binds to and stabilizes the open and active conformation of ezrin
    Article Snippet: Mycoplasma-free primary human glomerular endothelial cells (hGENs) were purchased from Angio-Proteomie (# cAP-0004) and maintained in EGM-2 MV Bulletkit growth media (# CC-3162, Lonza) containing 5% FBS and 1% penicillin/streptomycin at 37 °C in humidified air containing 5% CO 2 . .. Cells were cultured on Quick Coating Solution (Angio-Proteomie, # cAP-01). .. Cells in the logarithmic phase of growth were transfected using Lipofectamine 2000 (Invitrogen, # 11668-027) according to the manufacturer’s protocol.

    Article Title: CLIC5A binds to and stabilizes the open and active conformation of ezrin.
    Article Snippet: Mycoplasma-free primary human glomerular endothelial cells (hGEN) were purchased from Angio-Proteomie (# cAP-0004, Boston, MA) and maintained in EGM-2 MV Bulletkit growth media (# CC-3162, Lonza, Walkersville, MD) containing 5% FBS and 1% penicillin/streptomycin at 37°C in humidified air containing 5% CO2. .. Cells were cultured on Quick Coating Solution (Angio-Proteomie, # cAP-01, Boston, MA). .. Cells in the logarithmic phase of growth were transfected using Lipofectamine 2000 (Invitrogen, # 11668-027) according to the manufacturer’s protocol.

    Article Title: HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
    Article Snippet: Endothelial cells used were human pancreatic microvascular endothelial cells (HPaMEC) purchased from ScienCell Research Laboratories, and human umbilical vein endothelial cells (HUVEC, RRID: CVCL_9Q53) were purchased from ATCC. .. Endothelial cells were cultured in endothelial cell medium (ScienCell #1001) in flasks coated with fibronectin (2 μg/cm 2 ) or Quick Coating Solution (Angio-Proteomie #CAP-01). ..

    other:

    Article Title: Drug-free Immunotherapeutic Biomimetic Nanoparticles for Treating Triple-Negative Breast Cancer
    Article Snippet: The following materials and reagents were used in this study: Membrane Protein Extraction Kit (ProteoExtract), acetone, chloroform, methanol, DMSO, Tween 20, phosphate-buffered saline (PBS), RPMI-1640, DMEM, Eukitt (R) Quick-hardening mounting medium, 25 mm Sterile Syringe Filters, 0.02 μm PVDF, and cholesterol from Sigma-Aldrich-Merck; 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC), 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), and 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine- N -(lissamine rhodamine B sulfonyl) (PE-Rhod) from Avanti Polar Lipids, Inc.; dialysis tubes (12–14 kDa and 1000 kDa) from Repligen; Dynamic Light Scattering (DLS), ZetaSizer Nano, and disposable cuvettes for zeta potential measurements from Malvern Instruments; rabbit anti-CD11b (MAB11242–100) for Western blotting, and DuoSet ELISA kits for TNF-α, TGF-β, IFN-γ and IL-6 from R&D Systems; rabbit anti-CD68 (ab125212), rabbit anti-CD163 (ab182422), goat antirabbit IgG-HRP (ab6721), hematoxylin and eosin kit, and DAB substrate kit from Abcam; flow cytometry antibodies: anti-CD45-FITC (BLG-157608), anti-CD11b-Alexa Fluor 647 (BLG-101218), anti-F4/80-APC-Cy7 (BLG-157315), anti-Ly6G-BV711 (BLG-127643), anti-Ly6C-PE (BLG-128008), anti-CD3-Spark Blue 574 (BLG-100276), anti-CD8a-Alexa Fluor 594 (BLG-100758), anti-CD4-BV510 (BLG-100449), anti-FoxP3-Pacific Blue (BLG-126410), anti-CD19-PE-Cy7 (BLG-152418), and Zombie Violet Viability Kit from BioLegend; semi microvolume disposable polystcytokinesyrene cuvettes for size measurements and MycoStrip Mycoplasma Detection Kit from Tamar Ltd.; 5X Sample Buffer from A2S; Trans-Blot Turbo Mini PVDF membrane, TC20 Automated Cell Counter and slides, and Clarity Western ECL Substrate from Bio-Rad Laboratories; Quick Coating Solution from AngioProteomie; NanoAssemblr Benchtop and Microfluidic Cartridge from Precision Nanosystems (Cytiva); Infinite M Plex multimode microplate reader from Tecan; Pierce BCA kit,1-Step TMB ELISA Substrate Solutions, Vybrant DiD Cell-Labeling Solution, Halt protease inhibitor cocktail, Quant-iT Endotoxin Detection Assay Kit ( Q32892 ) and wheat germ agglutinin-Alexa Fluor 488 from ThermoFisher Scientific; μ-slide 0.4 was purchased from ibidi, and Endothelial Cell Medium with supplements from ScienCell; InVivo mAb antimouse PD-1 (CD279) (BE0146–5A) from BioXCell.

    Article Title: Drug-free Immunotherapeutic Biomimetic Nanoparticles for Treating Triple-Negative Breast Cancer.
    Article Snippet: The following materials and reagents were used in this study: Membrane Protein Extraction Kit (ProteoExtract), acetone, chloroform, methanol, DMSO, Tween 20, phosphate-buffered saline (PBS), RPMI-1640, DMEM, Eukitt(R) Quick-hardening mounting medium, 25 mm Sterile Syringe Filters, 0.02 μm PVDF, and cholesterol from Sigma-Aldrich-Merck; 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (PE-Rhod) from Avanti Polar Lipids, Inc.; dialysis tubes (12−14 kDa and 1000 kDa) from Repligen; Dynamic Light Scattering (DLS), ZetaSizer Nano, and disposable cuvettes for zeta potential measurements from Malvern Instruments; rabbit antiCD11b (MAB11242−100) for Western blotting, and DuoSet ELISA kits for TNF-α, TGF-β, IFN-γ and IL-6 from R&D Systems; rabbit antiCD68 (ab125212), rabbit anti-CD163 (ab182422), goat antirabbit IgG-HRP (ab6721), hematoxylin and eosin kit, and DAB substrate kit from Abcam; flow cytometry antibodies: anti-CD45-FITC (BLG157608), anti-CD11b-Alexa Fluor 647 (BLG-101218), anti-F4/80APC-Cy7 (BLG-157315), anti-Ly6G-BV711 (BLG-127643), antiLy6C-PE (BLG-128008), anti-CD3-Spark Blue 574 (BLG-100276), anti-CD8a-Alexa Fluor 594 (BLG-100758), anti-CD4-BV510 (BLG100449), anti-FoxP3-Pacific Blue (BLG-126410), anti-CD19-PE-Cy7 (BLG-152418), and Zombie Violet Viability Kit from BioLegend; semi microvolume disposable polystcytokinesyrene cuvettes for size measurements and MycoStrip Mycoplasma Detection Kit from Tamar Ltd.; 5X Sample Buffer from A2S; Trans-Blot Turbo Mini PVDF membrane, TC20 Automated Cell Counter and slides, and Clarity Western ECL Substrate from Bio-Rad Laboratories; Quick Coating Solution from AngioProteomie; NanoAssemblr Benchtop and Microfluidic Cartridge from Precision Nanosystems (Cytiva); Infinite M Plex multimode microplate reader from Tecan; Pierce BCA kit,1Step TMB ELISA Substrate Solutions, Vybrant DiD Cell-Labeling Solution, Halt protease inhibitor cocktail, Quant-iT Endotoxin Detection Assay Kit (Q32892) and wheat germ agglutinin-Alexa Fluor 488 from ThermoFisher Scientific; μ-slide 0.4 was purchased from ibidi, and Endothelial Cell Medium with supplements from ScienCell; InVivomAb antimouse PD-1 (CD279) (BE0146−5A) from BioXCell.

    Membrane:

    Article Title: Impact of Dapagliflozin on insulin signaling and neuronal survival in stretch-induced traumatic brain injury model.
    Article Snippet: .. The PDMS membrane was pre-treated with a quick coating solution of collagen I (ANGIO-PROTEOMIE, cAP-01, sourced from porcine) prior to seeding the cells at a density of 1.5 × 104 cells per cm2. ..



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